A new case study is presented on our website going in-depth on the biochemical properties of Bruton’s tyrosine kinase (BTK) inhibitors using enzymatic activity assays and surface plasmon resonance (SPR). Using these techniques, the clinical stage inhibitor fenebrutinib is shown to have a unique preference for binding to non-phosphorylated BTK compared to its phosphorylated counterpart. Key features of this case study include the comparison of full-length BTK and the BTK kinase domain in enzymatic assays, which differ in their ability to be autophosphorylated, and the comprehensive analysis of SPR binding curves using an alternative binding model.

NTRC is a precision medicine company dedicated to discovering new anti cancer drug candidates. We help you to find a mechanistic hypothesis before entering the clinic. We can study a wide range of cancer cells, primary patient material and immune cells in vitro, in isolation and in coculture, after exposure to monotherapy and combination therapy. In addition, we perform in-depth mechanistic analyses in cells and by biophysical methods, such as Biacore and LC-MS/MS. Keywords are: Quality. Flexibility. Short Turnaround Time.