Shipment of Your Compounds
Oncolines B.V.
Attn. Mrs. N. Willemsen-Seegers
Industrielaan 63
5349 AE Oss
The Netherlands
Tel.: +31 412 700 501
Mail: services@oncolines.com
Your shipper should be able to advise. Description of the materials should be on the Airway Bill.
Description of the materials: “Novel research compound for NON-human research”.
Please add MSDS (Safety Sheet).
You can ship your compound at any time. The turnaround time starts at arrival of the compounds at Oncolines.
Deliverables
The study results are owned by the Client. We destroy study related material after a pre-defined period.
ResidenceTimer™
The affinity is the most precise determination of the interaction between a drug and its target. It is the ratio between the dissociation and association rate constants. In contrast to an SPR-based affinity constant, a biochemical activity-based IC50 may be an underestimation of the real binding affinity. This is due to a lower limit of detection in biochemical activity assays which is dependent on the enzyme concentration.
Target residence time and half-life are calculated from the dissociation rate.
The compound response will be subtracted with both the reference channel response and the blank injection (double referencing).
We can work with, for instance, up to 5% DMSO. The standard concentration is 1% DMSO.
We can work with a variety of chips, for instance Ni-NTA chips for his-tagged proteins, protein A chips for antibodies, and CM5 chips for untagged proteins.
Yes, we can also perform assays that are different from the experiments described on our website.
It is recommended to choose a range that includes the biochemical potency (IC50) of the compound at the lower part of the test concentration range. For example, for a compound with an IC50 of 3 nM, we would advise to use a range of 1 -100 nM.
In principle the quote is prepared for reversible compounds. In the case of irreversible compounds, additional material costs (protein and chip) apply.
If you are uncertain about the concentration range, we recommend to start with a relatively high concentration range.
We can be flexible and can increase the time of the dissociation phase if desired.
QuickScout™
You can cherry-pick the kinases of your interest, – you can even select just one kinase for profiling.
The serine/threonine (STK) panel represents a variety of groups, with the exception of tyrosine kinases.
ELISA assay request is acceptable if your target is listed on Carna’s QSS Assist™ ELISA Assay Kit. Detailed information targets can be requested via the Contact Form (below) or services@oncolines.com.
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Indeed, high-throughput screening of more than 100,000 compounds is offered via the Mobility Shift Assay.
The service incorporates a 30 minute pre-incubation at room temperature with the test compound(s) prior to measuring the activity in our standard Mobility Shift Assay.
Yes, you can contact Carna Biosciences directly via carnabio.com. However, European clients will be kindly redirected to Oncolines, since we represent Carna for Europe. So, if you are from Europe, please contact us via the Contact Form, send a mail to services@oncolines.com or call us at: +31 412 700 501.
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It can be done either way, whatever you prefer. The shipment address of Oncolines is:
Oncolines B.V.
Attn. Mrs. N. Willemsen-Seegers
Building OP
Kloosterstraat 9
5349 AB Oss
The Netherlands
Tel.: +31 412 700 501
Mail: services@oncolines.com
The shipment address of Carna is:
Carna Bioscience, Inc.
Attn. Dr. Yusuke Kawase
Sales and Marketing
BMA 3F 1-5-5 Minatojima-Minamimachi
Chuo-ku, Kobe 650-0047
Japan
Tel.: +81 78 302 7091
Mail: yusuke.kawase@carnabio.com
Submission in 96-well plates with columns 1 & 2 empty is preferred, but tubes and 384-well plates are also acceptable. Compound preparation conditions: 100% DMSO solution of 100 x the top concentration.
We use semi-log dilution series to generate 10 point curves, all compound concentrations are assayed in duplicate.
The 1 mM ATP profiling assay is useful in determining the potential selectivity of your inhibitor using conditions approaching physiological ATP concentrations. The 1 mM ATP assay can also help identify possible allosteric inhibitors when IC50 data generated at ATP Km is also available.
Results are provided in Excel format. Interim data is uploaded to a secured website within 1 week of initiating your study and the Final report is emailed to you upon completion of the study. A PDF report describing the study is sent via email and in printed form upon completion of the project.