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FAQ2021-03-24T15:18:50+01:00

Shipment of Your Compounds

What is the shipping address for compounds?2021-09-07T11:08:28+02:00

Oncolines B.V.
Attn. Mrs. N. Willemsen-Seegers
Industrielaan 63
5349 AE Oss
The Netherlands
Tel.: +31 412 700 501
Mail: services@oncolines.com

Do we need to include a shipment declaration for customs?2020-07-02T15:20:44+02:00

Your shipper should be able to advise. Description of the materials should be on the Airway Bill.
Description of the materials: “Novel research compound for NON-human research”.
Please add MSDS (Safety Sheet).

Are there timeslots for shipment of compounds?2021-09-10T22:01:34+02:00

You can ship your compound at any time. The turnaround time starts at arrival of the compounds at Oncolines.

Deliverables

What is the status of IP that is generated during the studies?2021-09-10T22:00:23+02:00

The study results are owned by the Client. We destroy study related material after a pre-defined period.

ResidenceTimer™

What is the meaning of affinity?2020-07-17T10:28:49+02:00

The affinity is the most precise determination of the interaction between a drug and its target. It is the ratio between the dissociation and association rate constants. In contrast to an SPR-based affinity constant, a biochemical activity-based IC50 may be an underestimation of the real binding affinity. This is due to a lower limit of detection in biochemical activity assays which is dependent on the enzyme concentration.

How are target residence time and the half-life determined?2020-07-02T15:28:32+02:00

Target residence time and half-life are calculated from the dissociation rate.

Do you correct for background signals during the experiments?2020-07-02T15:29:08+02:00

The compound response will be subtracted with both the reference channel response and the blank injection (double referencing).

Is it possible to add organic solvent in the buffer if this is needed to dissolve the proteins?2020-07-02T15:29:32+02:00

We can work with, for instance, up to 5% DMSO. The standard concentration is 1% DMSO.

Could you also measure non-biotinylated proteins, for instance using a CM5 chip?2020-07-02T15:29:58+02:00

We can work with a variety of chips, for instance Ni-NTA chips for his-tagged proteins, protein A chips for antibodies, and CM5 chips for untagged proteins.

Can you perform a Biacore experiment based on a protocol that we will send you?2020-07-17T10:30:24+02:00

Yes, we can also perform assays that are different from the experiments described on our website.

What is the recommended concentration range to use for the compounds?2021-03-25T21:19:48+01:00

It is recommended to choose a range that includes the biochemical potency (IC50) of the compound at the lower part of the test concentration range. For example, for a compound with an IC50 of 3 nM, we would advise to use a range of 1 -100 nM.

How do you prepare a quote when it is unknown if a compound is reversible or irreversible?2020-07-17T10:32:15+02:00

In principle the quote is prepared for reversible compounds. In the case of irreversible compounds, additional material costs (protein and chip) apply.

What will you do when the range of a compound concentration is not optimal and an additional test is needed?2020-07-17T10:33:17+02:00

If you are uncertain about the concentration range, we recommend to start with a relatively high concentration range.

Do you apply a fixed time for the dissociation phase?2020-07-02T15:32:25+02:00

We can be flexible and can increase the time of the dissociation phase if desired.

QuickScout™

Is it possible to select specific kinases for testing, or should we choose from the full panel, or one of the TK, STK, and cell-cycle panels?2021-03-25T21:20:36+01:00

You can cherry-pick the kinases of your interest, – you can even select just one kinase for profiling.

Do you have a pre-defined panel that is comprised of representative kinases from different groups?2020-07-02T15:37:42+02:00

The serine/threonine (STK) panel represents a variety of groups, with the exception of tyrosine kinases.

I see references to mobility shift assay and IMAP™. Do you also offer ELISA assays?2021-09-10T21:59:10+02:00

ELISA assay request is acceptable if your target is listed on Carna’s QSS Assist™ ELISA Assay Kit. Detailed information targets can be requested via the Contact Form (below) or services@oncolines.com.

  • This field is for validation purposes and should be left unchanged.
Do you offer high-throughput screening via mobility shift assay?2021-03-25T21:21:20+01:00

Indeed, high-throughput screening of more than 100,000 compounds is offered via the Mobility Shift Assay.

How long is the pre-incubation time in the mobility shift assay?2021-03-25T21:21:31+01:00

The service incorporates a 30 minute pre-incubation at room temperature with the test compound(s) prior to measuring the activity in our standard Mobility Shift Assay.

The service is offered through your partner, Carna Biosciences, Inc.. Does this mean that I can also contact them directly?2021-09-29T14:30:39+02:00

Yes, you can contact Carna Biosciences directly via carnabio.com. However, European clients will be kindly redirected to Oncolines, since we represent Carna for Europe. So, if you are from Europe, please contact us via the Contact Form, send a mail to services@oncolines.com or call us at: +31 412 700 501.

  • This field is for validation purposes and should be left unchanged.
Since you are the European representative, should we ship the compounds to Oncolines B.V. or directly to Carna Biosciences?2021-09-07T16:20:28+02:00

It can be done either way, whatever you prefer. The shipment address of Oncolines is:

Oncolines B.V.
Attn. Mrs. N. Willemsen-Seegers
Industrielaan 63
5349 AE Oss
The Netherlands
Tel.: +31 412 700 501
Mail: services@oncolines.com

The shipment address of Carna is:

Carna Bioscience, Inc.
Attn. Dr. Yusuke Kawase
Sales and Marketing
BMA 3F 1-5-5 Minatojima-Minamimachi
Chuo-ku, Kobe 650-0047
Japan
Tel.: +81 78 302 7091
Mail: yusuke.kawase@carnabio.com

What is the required mode of submitting compounds for profiling and dose response?2020-07-17T10:53:05+02:00

Submission in 96 well plates with columns 1 & 2 empty are preferred but acceptable with tubes and 384 well plates. Compound preparation conditions : 100% DMSO solution of 100 x the top concentration.

What is the dilution series for dose response curves?2020-07-17T10:52:52+02:00

We use semi-log dilution series to generate 10 point curves, all compound concentrations are assayed in duplicate.

What is the utility of profiling at 1mM ATP?2021-03-25T21:22:45+01:00

The 1mM ATP profiling assay is useful in determining the potential selectivity of your inhibitor using conditions approaching physiological ATP concentrations.   The 1mM ATP assay can also help identify possible allosteric inhibitors when IC50 data generated at ATP Km is also available.

What report format is used to present assay results on my compounds?2020-07-17T10:57:53+02:00

Results are provided in Excel format. Interim data is uploaded to a secured website within 1 week of initiating your study and the Final report is emailed to you upon completion of the study. A PDF report describing the study is sent via email and in printed form upon completion of the project.

Contact us

Contact us:2021-09-09T11:14:06+02:00

+31 412 700 501
 
Send e-mail to services@oncolines.com

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