Molecular Interactions – Assay Kits
Homogeneous Mix-and-Measure Assay Kits for Cancer Immunotherapy
Biochemical High-Throughput Screening of Cancer Immunotherapy Drug Candidates
Activity of Tryptophan-Metabolizing Enzymes Using NFK Green™ -GreenScreen™
Fluorescence Assay for the Evaluation of Human and Mouse IDO1 and TDO Activity
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Catalog No.
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Datapoints | Cell-based | Biochemical | Contents |
|---|---|---|---|---|
| NTRC-GSCell-1K | 1,000 | Probe; substrate; IDO1 and TDO reference inhibitors | ||
| NTRC-GSCell-10K | 10,000 | idem | ||
| NTRC-hIDO-1K | 1,000 | Probe; substrate; assay buffer; human IDO1 protein; IDO1 reference inhibitor | ||
| NTRC-hIDO-10K | 10,000 | idem | ||
| NTRC-mIDO-1K | 1,000 | Probe; substrate; assay buffer; mouse IDO1 protein; IDO1 reference inhibitor | ||
| NTRC-hTDO-1K | 1,000 | Probe; substrate; assay buffer; human TDO protein; TDO reference inhibitor | ||
| NTRC-hTDO-10K | 10,000 | idem | ||
| NTRC-mTDO-1K | 1,000 | Probe; substrate; assay buffer; mouse TDO protein; TDO reference inhibitor |
Key Features |
NFK Green™ |
|---|---|
| Mild reaction conditions | |
| Ambient temperature | |
| Neutral pH | |
| Homogeneous assay format | |
| HTS compatible | |
| End-point measurements | |
| Fluorescent readout |
Tryptophan metabolism plays an important role in immune modulation and neurodegenerative disease. Tryptophan-metabolizing enzymes are drug targets for cancer immunotherapy and neurodegenerative disease. We have developed NFK Green™ assays for the high-throughput screening of the tryptophan-metabolizing enzymes indoleamine 2,3-dioxygenase (IDO1) and tryptophan dioxygenase (TDO). The NFK Green™ assay works under mild conditions and is suitable for high-throughput screening. The fluorescence assay is based on a highly specific and unique label, developed by NTRC, named NFK Green™. The protocol allows a rapid implementation of the assay for high-throughput screening or regular compound testing.
Schematic representation of NFK Green™ assay. NFK Green™ reacts with NFK to form a green fluorescent molecule, with an excitation wavelength of 400 nm and an emission wavelength of 510 nm. At this wavelength, fluorescence interference of library compounds is minimal.
References
Seegers et al. (2014) High-throughput fluorescence-based screening assays for tryptophan-catabolizing enzymes. Journal of Biomolecular Screening, 19 (9):1266-1274.
